This study focuses on development of an interface for gesture control and Boolean operation of 3D object in 3D computer-aided design (CAD). Although mouse and keyboard have been mainly used as computer input devices, novel computer interfaces are becoming increasingly popular with rapid development of computer technologies. Among several computer interfaces, gesture interface is well known as an operable system through natural human behavior. However, the application to 3D CAD has been scarcely studied so far. From the backgrounds, a simple gesture interface was developed to operate a 3D object in 3D CAD. Leap Motion Controller (LMC) was used as a motion sensor device. Dialog-based graphical user interface (GUI) was also created for the parameter setting. As a result, several operational functions could be successfully executed through various hand-finger motions. Moreover, the system enabled us to experience an intuitive interaction with 3D CAD. Then, the several demonstrations were performed to identify the recognition characteristics of gesture interface. The differences of recognition rate were revealed as the results. Especially, it confirmed that a combination of static and dynamic gestures could help to enhance the recognition rate in gesture interface using LMC. In accordance with the findings, the functions for Boolean operation were also implemented for 3D object’s manipulation such as extrusion and hole making.