In this work, an electrophoretic deposition (EPD) method was utilized to cover a biocompatible stainless steel medical grid type 316L with nanoparticles of hydroxyapatite (HA). ln conjunction with the biopolymer chitosan (CHT), as a binder, it enhances the substrate's adhesive capabilities. The chosen bio-coating method is the EPD process of 316L Stainless Steel alloy due to its ease of use, low cost, and capacity to coat intricate items. Consequently, this research has studied different concentrations of the materials utilized, the most significant variables, and their effects in order to acquire the optimal attributes for the coating layer in the present research's goal, using voltage applied and deposition time. The coating periods were 2, 4 and 6 minutes, and the concentrations were 4, 7 and 10 g/L, while the voltages were modulated between 20, 40, and 60 volts. In spite of the fact that the metallic medical applications and bone replacement have made substantial and fruitful progress, it's not over yet; difficulties persist. The coating procedures have a significant impact on how well the composite materials work in the biological devices. To improve the properties of composites used in biomedicine, coating them is an essential step. X-ray diffraction (XRD) spectroscopy and scanning electron microscopy (SEM) were used to examine the deposited coatings, and the Zeta potential for suspensions was computed.