Sustainable construction represents a pivotal aspect of contemporary engineering endeavors, which are directed towards the minimization of the detrimental impact of the construction sector on the environment. This is achieved through the efficient utilization of resources and the reduction of waste generation. The study presented an analysis of the mechanical properties of ad hoc reinforced concrete beams made of concrete with recycled and natural aggregate. The objective of this study was to assess the potential of recycled aggregates as a substitute for natural aggregates in reinforced concrete structures, with the aim of contributing to the sustainable development of construction. The experiments compared the behavior of reinforced concrete beams with the same degree of reinforcement but differing in the type of aggregate. The results demonstrated that recycled aggregate beams exhibited lower stiffness and higher deformation under load compared to natural aggregate beams, particularly at loads close to the breaking force. Nevertheless, similar failure modes and cracking patterns were observed in both types of beams, indicating that concrete with recycled aggregate could be employed effectively in specific structural applications.