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THE TECHNIQUE OF FLOW CYTOMETRY IN DIAGNOSTIC RESEARCH
 
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1
Department of Hematooncology and Bone Marrow Transplantation, Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, Poland
2
Students Scientific Association at Department of Hematooncology and Bone Marrow Transplantation Medical University of Lublin, Al. Racławickie 1, 20-059 Lublin, Poland
Publication date: 2013-09-06
 
Adv. Sci. Technol. Res. J. 2013; 7(19):55–59
 
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ABSTRACT
Flow cytometry is a technology that simultaneously counts and then examines multiple physical and/or chemical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell scatters incident laser light and emits fluorescence. One of the most significant applications is immunophenotyping of cells - the most important tool in diagnosis and monitoring haematological disorders, such as acute and chronic leukemia, lymphoma, monoclonal gammopathy, myelodisplastic and myeloproliferative diseases.