Nanotechnology is one of the most important research areas and is presented in the vast fields of knowledge such as road construction industry has been surrounded. This paper has focused on the potential benefits of nano particles for modification of asphalt mixtures. Nano Al₂O₃ due to its unique properties can improve the dynamic characteristics of hot mix asphalt. Fatigue life of asphaltic samples is determined with indirect tensile fatigue tests by using of UTM. The results show that fatigue life of modified asphalt mixtures in compare with conventional mix is significantly increased. Beside, based on experimental results and numerical analyses, a new model is presented for prediction the fatigue behavior of asphalt mixtures modified with nano Al₂O₃. This model can completely characterized the fatigue performance of modified asphalt under dynamic loading conditions and different temperatures.